CLSI H 21-A5 PDF

CLSI HA5 Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays and Molecular Hemostasis Assays;. CLSI HA5. Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays; Approved Guideline. HA5 does not provide general guidelines for the performance of coagulation testing. Performance guidelines for specific coagulation assays are addressed.

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Whole 21-q5 Analyses S5. Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories. Introduction Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories. Enzymatic – release of enzymes which degrade analytes e. A survey of Australian Laboratories enrolled in the RCPA Quality Assurance Programs confirmed an ongoing variation in the management and reporting of haemolysed samples.

Find articles by Ken Sikaris. The interference can be the result of various mechanisms including:. Standards Subscriptions from ANSI provides a money-saving, multi-user solution for accessing standards. Please first log in with a verified email before subscribing to alerts.

Clinically relevant limits can be sourced from: If a laboratory decides to report affected tests, the H-Index levels used for these purposes must be documented.

Journal List Clin Biochem Rev v.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

However, these guidelines may also be considered for other sample types e. This decision should be guided by a policy developed in consultation with requesting clinicians. Laboratories must monitor the frequency of haemolysed blood samples and investigate the source of collection to enable continuous improvement. If the document is revised or amended, you will be notified by email.

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Published clinical outcome studies describing performance requirements for the specific analyte. Clin Chem Lab Med. Your Alert Profile lists the documents that will be monitored. Additive – as result of greater concentrations of analytes in cells than in plasma e. Statements of opinion clzi AACB publications are those of the contributors. This standard is not included in any packages. Author information Copyright and License information Disclaimer.

Observational study to determine factors associated with blood sample haemolysis in the emergency department.

As the voice of the U. Appendix Example of a Haemolysis Colour Chart which also shows haemolysis index concentration levels across different analytical platforms Courtesy Goce Dimeski, Pathology Queensland. The interference can be the result of various mechanisms including: Cut-offs at which assays are affected must be reviewed and updated when analytical platforms are changed.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

A clear statement describing the magnitude and direction of the interference must be included. Tests of the coagulation system are very sensitive to storage time and temperatureconcentration of anticoagulant, and surface of containers; attention to these parameters is important.

Open in a separate window. Best Practices in Phlebotomy. National Center for Biotechnology InformationU. Quantitative Estimation of Haemolysis S2. Haemolysis as influence and interference factor.

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Please review our privacy policy. The effect of hemolysis on current troponin assays confounding preanalytical variable? You can download and open this file to your own computer but DRM prevents opening this file on another computer, including a networked server. Subscription pricing is determined by: Best Practices in Phlebotomy Actions 21-x5 Response to Haemolysis S4. Already Subscribed to this document.

Impact of Haemolysis on Test Results S3. Dilutional gross haemolysis releasing cell fluid and content – leading to false lowering of results e.

Factors Affecting Haemolysis, specimencare. Standards Subsctiption may be the perfect solution. Laboratories must be familiar with best practice collection processes.

Example of a Haemolysis Colour Chart which also shows haemolysis index concentration levels across different analytical platforms Courtesy Goce Dimeski, Pathology Queensland. This consensus statement is primarily intended as a guideline for diagnostic pathology laboratories undertaking any analyses performed on serum or plasma samples.

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Similarly, brown coloured serum due to methaemalbumin, normal results for potassium or the same result from arterial and venous collections should prompt further investigation e.